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Production of microbial enzymes and their stabilization by encapsulation
Hazuchová, Eva ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The present thesis deals with the production of microbial enzymes and their subsequent stabilization through encapsulation. The theoretical part focuses on microbial enzymes, especially extracellular hydrolases, their producers and characteristics. Within the theory is also discussed the possibility of the application of enzymes in the field of pharmacy and medicine. Experimental work was focused on the actual production of microbial enzymes and methods for their to stabilization. The production of proteolytic and lipolytic enzymes in dependence on time and the used culture substrate were followed. The highest enzyme production was observed in Aspergillus oryzae when cultured on wheat bran at the third day of cultivation. In the experimental part was further carried out the identification, isolation and purification of enzymes. A substantial part of the experiment was to stabilize produced microbial enzymes by encapsulation. Enzymes were entrapped into alginate particles with encapsulation efficiency in the range of 55-70 %. The highest efficiency exhibited encapsulated enzymes from Aspergillus oryzae. Subsequently, long-term stability of the encapsulated enzyme in two environments (in water and gel) was followed during six weeks incomparison with free enzyme. During storage of free enzyme a significant decrease in enzyme activities occured, especially between the fourth and sixth week of storage. On the contrary, in encapsulated increased enzyme activities were observed. Empty particles exhibited higher stability during storage in the gel than in water. In this thesis potential use of enzymes in the pharmaceutical industry as agents promoting digestion was tested too. According to the results, particles with encapsulated microbial enzymes could be considered as suitable for some pharmaceutical applications.
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Equipment for sports and relaxation
Doupal, Ondřej ; Bláhová, Pavla (referee) ; Čuprová, Danuše (advisor)
The project design processes multifunctional center for leisure and sports. The center is situated in Šternberk and its capacity is about 150 people. There is inter alia the wellness center, with saunas and whirlpool, a fitness, gym and a beauty salon. Architecturally, the object is conceived as paralell blending of three units of a different colors and sizes. As well as all construction compositions are more developed basis for air conditioning systems. Documentation is processed at the level of documentation for building permit.
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Aronia as a raw material for cosmetic production
Palkovská, Kateřina ; Sýkora, Michal (referee) ; Zemanová, Jana (advisor)
This bachelor thesis deals with extraction of polyphenols from fruits and leaves of aronia and consequent use of obtained extract in cosmetic production. In the theoretical part are information about the origin of the aronia, the botanical characteristics of Aronia Melanocarpa and about its utilization. Furthermore, the chemical composition of the plant material of the aronia is described, which is also common for fruits. At the end of the theoretical part are described extraction and separation techniques, methods of determination of total polyphenolic compounds and brief characteristics of the material safety data sheet. The experimental part describes the optimization of the prepared extract so that the extract contains as many polyphenolic compounds as possible. 50% ethanol at room temperature was shown to be optimal for leaf and fruit extraction, and the maceration time was found to have a significant effect on the polyphenolic compounds obtained. For the obtained optimized macerate, some chemical and physical properties such as pH, density, refractive index were determined for the purpose of producing a material safety data sheet and product specification. At the end of the experimental part were prepared cosmetic products with the addition of macerate from crushed aronia fruits.
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Business Plan for Hairdressing and Cosmetics Studio
Chvátalová, Lucie ; Kaňovská, Lucie (referee) ; Chlebovský, Vít (advisor)
This diploma thesis is focused on creating a business plan for starting the new hairdresser‘s and beauty studio. The appropriate strategies and tools will be determined on the basis of the analysis of external, industrial, internal environment and marketing research which make studio to undertake their activities, meet the customer’s needs and profit. Feasebility and competetivness of the business evalution are parts of this thesis.
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Study of particles of various cosmetic pigments
Cerevatova, Kristina ; Opravil, Tomáš (referee) ; Zemanová, Jana (advisor)
The main focus areas of this master thesis include the writing of a literature review, which contains an overview and segregation of pigments which are used in the preparation of cosmetic products. The thesis is focused on the properties and characterization of pigments for use in cosmetology. The following focus is on legislation, more specifically on pigments that the cosmetic industry allows, limits and does not allow. The main part of this thesis is an overview of methods for the characterization of pigments. The practical part deals with the characterization of particles of selected cosmetic pigments by applying methods for determination of color, elemental and phase composition, particle size and morphology. The thesis is concluded by evaluation of the achieved results.
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Study of preservative systems for cosmetics
Šmétková, Zuzana ; Vítová, Eva (referee) ; Zemanová, Jana (advisor)
The aim of this diploma thesis was to prove the antimicrobial actvivity of the selected syntetic preservatives used in cosmetics, such as methylparaben, butylparaben, phenoxyethanol, bronopol, imidazolidinyl urea and methylisothaizolinone, in different concentrations (up to the highest permitted concentration and in increased concentrations), and of some essential oils, which are possible to use in cosmetics (essential oil from Eucalyptus globulus, Pimpinella anisum, Lavandula officinalis, Rosmarinus officinalis, Foeniculum vulgare, Mentha piperita, Juniperus communis and Pinus sylvestris). The effect was investigated in every preservative system alone, in order to evaluate the spectrum and the rate of each one. As indicator microorganism was chosen Bacillus subtilis and Penicillium chrysogenum. The used methods was disk difusion method and modify difusion method. From synthetic preservatives had the best effects bronopol, imidazolidinyl urea and methylisothiazolinone. From chosen natural antimicrobails had the effect against both indicator microorganisms the Mentha piperita, Foeniculum vulgare and Juniperus communis extracts.
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Polyfunctional residential building
Smetanová, Lenka ; Gintar, Jan (referee) ; Petříček, Tomáš (advisor)
The subject of the master´s thesis is a design of polyfunctional residential building. The residential building is partly basament and has four floors and ten flats. Two flats are maisonettes. On the first floor are a cosmetics salon and a massages salon. The thesis contains a dispositional and building construction design and elaboration of the given part of implementary project documentation.
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Determination of alergenic and potential alergenic metals in cosmetics
Krakovková, Lenka ; Zemanová, Jana (referee) ; Diviš, Pavel (advisor)
The aim of the diploma thesis was to provide an overview of the prevalence of allergenic and potentially allergenic metals in eye shadows. The diploma thesis gives an overview of the legislation on cosmetics and description of the types of allergenic reactions caused by allergenic and potential allergenic metals in eye shadows. Listed below are the preparation methods of the samples for analysis and the list of the methods by which can the selected metals be analyzed. The experimental part of the diploma thesis deals with an analysis of selected allergenic and potentially allergenic metals in eye shadows. In the experimental part of diploma thesis method of sample preparation for analysis of eye shadows and a method of analysis of sample of eye shadows by ICP-MS have been optimized. Monitored analytes were selected metals. Altogether 6 samples of eye shadows from different manufacturers were chosen. Measured results have been statistically processed, confronted with the applicable legislation and assessed in terms of possible allergic reactions.
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Fragrance Allergens in Foods and Everyday Use Products
Divišová, Radka ; Buňka, František (referee) ; Hojerova,, Jarmila (referee) ; Omelková, Jiřina (advisor)
Fragrances are added to food, cosmetics and other products primarily for enhancement of their sensory quality (taste and/or aroma). However, the usage of these compounds is associated with the wide range of various adverse biological effects. Some fragrances widely used in cosmetics are proved to cause e.g. skin sensitization, rashes, dermatitis, headache, cough etc. To protect the health of consumers, European union approved the cosmetic directive (ES/1223/2009) that requires the labeling of 26 allergen fragrances on the final product label, if concentration exceeds the given level (0,01 % for rinse-off and 0,001 % for leave-on product). However, some producers hide the presence of allergens in the final product under the general term „aroma“. For this reason it is very important to monitor the content of these substances in cosmetic products. Fragrances are found not only in cosmetics, but the various types of food are also aromatized by them. Unlike cosmetics, the food producers are not obliged to label the presence of fragrance allergens on the packaging. Therefore, the monitoring of these fragrances is highly desirable because of the potential health risks they pose. The aim of this study was to develop a method for simultaneous determination of regulated fragrance allergens in food and everyday use products. The method based on extraction of analytes by solid phase microextraction coupled to gas chromatography with FID detection (HS-SPME-GC-FID) was chosen on the basis of the literature review. The fiber CAR/PDMS provided the highest extraction efficiency among the SPME fibers tested. Univariate and multivariate data analysis were used to optimize the main parameters affecting microextraction process. The final method validation was performed in terms of linearity, repeatability, reproducibility, recovery, limits of detection and quantification. The optimized and validated method was applied to a wide range of products including cosmetics, aromatized food and fragranced toys. These products were also subjected to sensory evaluation especially in terms of taste and/or aroma (i.e. flavour), which may be associated with the content of the monitored fragrances. The profile test (EN ISO 13299) and seven-point category ordinal scale (ISO 4121) were used for sensory evaluation.
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Use of antimicrobial products of animal origin in cosmetics
Puškárová, Radka ; Bokrová, Jitka (referee) ; Márová, Ivana (advisor)
The Bachelor thesis deals with characterization a whey in free and encapsulated form and its application to cosmetic industry. The theoretical part is focused on characterization of whey, especially on its antimicrobial properties, and use of this effect in selected cosmetics preparatives. Further, methods of encapsulation and particles characterization were described. In the experimental part, antimicrobial properties of whey were tested by two different methods. Whey was encapsulated into three different types of particles: liposomes, alginate and chitosan. Long-term colloid stability of particles during one and three weeks of storage in model conditions was tested. Further, particles were added into model skin creams. Long-term sedimentation stability of these emulsions was analyzed by analytical centrifuge.
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